Practical aspects of Single Molecule Localization Microscopy
Prof. Christian Soeller & Dr. Alex Clowsley
University of Bern, Switzerland
In this webinar we will briefly cover some of the basics of SMLM with dSTORM and DNA-PAINT with a focus on practical aspects to obtain high quality data suitable for biological interpretation. This includes buffer choices for dSTORM and practical aspects of using DNA-PAINT in terms of sequence choices and rarely discussed issues. In our hands we find the use of test and reference samples important for quality assurance and will give some recommendations for robust sample selection and preparation. Software can be both an enabling and limiting factor of SMLM and we will mention our experiences in this area and share the qualities we as users would like to see across platforms. Finally, we will cover practical strategies for multi-colour and multi-channel SMLM as well as recently introduced proximity methods in SMLM to detect protein-protein interactions. Some illustrations from our own biological imaging data will be provided.