The inner life of integrin adhesion sites: From single molecules to functional macromolecular complexes
Dr. Gregory Giannone & Dr. Olivier Rossier
University of Bordeaux
Cells are mechanical living machines that remodel and move within their microenvironment by adhering and generating forces using integrin-dependent adhesion sites. Integrin adhesions initiate chemical and mechanical signals involved in cell migration, proliferation and differentiation. Using recent advances in super-resolution microscopy and single protein tracking, it is now possible to localize single proteins in three dimensions inside integrin adhesions, determine their diffusive behaviors, and mechanical responses during cell stretching. Here, we will present how we have used and developed tools to link the molecular behavior of integrin adhesion proteins with their functions during integrin activation and mechano-sensing.
Further introductory reading:
1. T. Orré, O. Rossier, G. Giannone, Exp. Cell Res. 379, 235–244 (2019).
2. A. Mehidi. et al., Current Biology 29, 2852-2866.e5 (2019).
3. M. Paszek et al., Nature. 511, 319–325 (2014).
4. O. Rossier et al., Nature Cell Biology. 14, 1057–1067 (2012).
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