Which fixation methods do you recommend for STORM imaging?
The best preservation of ultrastructure is in most cases obtained with chemical fixation with aldehyde (PFA, glutaraldehyde). However, in some cases, we recommend organic solvents (methanol). The choice is highly contingent on the structure of interest and, in the case of immunostaining, on the antibodies used. Thus the optimal fixation method needs to be optimized case by case.