Smart Flow

Automated workflow for Sample Preparation

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>NEW<  Abbelight Smart Flow is the easy-to-use automated workflow system dedicated to the accurate and reproducible preparation of samples for fluorescence microscopy. With Smart Flow and the Abbelight range of reagents, researchers have now a turnkey solution to save time and stay focused on their investigations.

Fits any experimental design for accurate and reproducible preparation of samples for fluorescence microscopy


Intuitive, time-saving, and easy to use: Individual methods can be created, saved, and recalled quickly for a run


Enables long-term perfusion studies such as multicolor PAINT imaging


SAFe reagents

optimized super-resolution buffers

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SAFe reagents are the solution you need to accelerate your research with Single Molecule Localization Microscopy.

These solutions are ready-to-use, easy to use, and optimized.

The Smart Kit is a dedicated buffer optimized for dSTORM imaging and compatible with a wide range of commercial dyes. The blinking pad kit will facilitate your non-adherent cell imaging experience. Freeze them with our solution for dSTORM imaging as well as regular and PALM microscopy.

Smart kit for STORM

Mounting buffer for STORM imaging

  • 10 doses per box
  • 200 µL per dose
  • 30 sec prepartion
  • 2 months in a fridge
  • 2 weeks on sample
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Compatible dyes

  • Phalloidin-AF ® 488, WGA-AF ® 488
  • AF ® 532, CF ® 532, Cy3b
  • AF ® 555, CF ® 555, AF ® 568, CF ® 568, Cy5, MemBrite™ 568
  • AF ® 647, CF ® 647, CF ® 658,CF ® 660, AF ® 680, CF ® 680, MemBrite™ 640

Blinking Pad kit for STORM

Imaging kit for cell immobilization and mounting in STORM buffer
  • 10 doses
  • 10 chamber slides
  • A detailed protocol
  • 30 min preparation
  • 2 months in a fridge
  • 2 weeks on sample
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Compatible samples

Non-adherent cells or structures:

  • Eukaryotic cells
  • Yeast cells
  • Bacteria
  • Viruses
  • Giant Unilamellar Vesicles
  • Beads

Pad kit for Widefield, TIRF microscopy and PALM

Imaging kit for cell immobilization and mounting 

  • 10 doses
  • 10 chamber slides
  • A detailed protocol
  • 30 min preparation
  • 2 months in a fridge
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Compatible samples


Non-adherent live or fixed cells:

  • Eukaryotic cells
  • Yeast cells
  • Bacteria
  • Viruses

SMART Staining kit

Fixation, Labeling and Imaging all in one kit !

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Fixation and immuno-staining are essential steps to ensure high-quality STORM acquisitions.

However, protocols and their optimization are often time-consuming, cumbersome, and require strong expertise in sample preparation.

To simplify this process, abbelight has developed the first ready-to-use sample preparation kit designed and optimized for STORM imaging: the SMART Staining Kit

Smart Staining kit

It is a turnkey solution designed and optimized for the fixation, labeling and imaging steps of 20 samples (20 mL). It is easy and fast to prepare, compatible with STORM imaging, and provides excellent sample quality with no background noise and can be stored up to 6 months at -20°C.
  • Pre-fixative solution
  • Fixative and post-fixative solutions
  • Neutralizing solutions
  • Blocking solution
  • Primary and secondary antibody solutions
  • Washing solutions


You have also the possibility to add :

  • Primary antibodies
  • Secondary antibodies
  • STORM buffer : Smart Kit or Blinking Pad Kit
  • Pad Kit


Customize your Abbelight Smart Staining Kit

  • Primary antibody : 
    •  anti-alpha-Tubulin (mouse)
    •  anti-Clathrin heavy Chain (mouse or rabbit)
    •  anti-TOM20, Mitochondria (mouse or rabbit)
  • Secondary antibody : 
    •  anti-mouse or anti-rabbit AF647, CF660 and CF680

*You can also customize up to 3 colors


SAFe samples

for calibration and training

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SAFe samples are dSTORM samples prepared by abbelight biologist for calibration and training on STORM imaging.

The following list shows you all cell types, cell structure, and fluorophores that can be used to prepare your sample!

Cell type

  • Cos7
  • U2OS
  • HeLa
  • HEK
  • Escherichia coli

Cell structure

  • microtubules
  • clathrin coated pits
  • mitochondria
  • bacterial cell wall and membrane


  • CF ® 568
  • AF ® 647, CF ® 647, CF ® 660, CF ® 680

A team of specialists
dedicated to your projects

Valentina Caorsi

Head R&D Imaging
Valentina has a PhD in Physics; she has nearly 15 years of research experience in biophysics developed between Genoa at the university of Genoa, London at Imperial College and Paris at Institut... ic_font_btn_plus Learn more

Cataldo Schietroma

Application scientist
Cataldo has a PhD in Biology and several years of experience in scientific research across diverse fields. After a post-doctoral position at UCLA, in the Departments of Molecular & Medical... ic_font_btn_plus Learn more

Cyndelia Guillaume

Product Sales Specialist
Graduated with a Master’s Degree in Cell Imaging from Rouen University, she’s double-skilled in Cell Biology and Optics, with particular experience in Single Molecule Localization Microscopy... ic_font_btn_plus Learn more
Meet the team