What the advantage of using Abbelight's system?

Abbelight’s module is a dual-view system that exploits supercritical angle fluorescence emission (Bourg et al. Nature Photonics 2015) and strong astigmatic PSF engineering to extract axial information (Cabriel et al. BiorXiv 2018). The combination of these two techniques is reference-free and does not require calibration (Cabriel et al. Optics Letters 2017). The localization precision is 15x15x15 nm3, the field of view is 200 x 200 um² and the imaging depth can go up to 5 um.