Detection and quantification of individual molecules in addition to nanometric spatial resolution

A new imaging paradigm has emerged

Nanoscopy, also called Single Molecule Localization Microscopy (SMLM) circumvents the diffraction limit of fluorescence microscopy, providing nanometric spatial super-resolution. This revolutionary breakthrough in imaging relies on the ability of photoswitching fluorescent dyes. These dyes can be toggled between emitting and non-emitting states, allowing precise localization of individual molecules with nanometer-scale accuracy. Because of its principle, SMLM stands out as the sole method capable of not only achieving such remarkable super-resolution but also quantifying and counting individual molecules within biological samples. SMLM stands as the pinnacle of simplicity and precision in imaging, rivaling electron microscopy in spatial resolution while offering a vastly more straightforward and accessible approach to unraveling the mysteries of the nanoscale world.

Nanoscopy specifically focuses on achieving high-resolution imaging at the nanometer scale, while microscopy encompasses a broader range of imaging techniques used for larger-scale objects

SMLM Techniques

Focus on your research, Abbelight masters and provides the entire value chain of the various SMLM methods: STORM, DNA-PAINT and PALM.

  • PALM
Abbelight pushes the limits of throughput even further with its innovations: ASTER, a patented uniform illumination and Smart multi-color detection strategy gives access to:
Uniform and homogeneous largest field of view
Simultaneous multicolor super resolution microscopy for an optimal imaging of multiple structures at the same time without any aberration
Ultimate 3D super-resolution providing access to best-in-class axial resolution


The largest, homogeneous field of view on the market

The patented Abbelight Adaptative Scanning for Tunable Excitation Rendering (ASTER) technology is a unique, versatile illumination scheme that creates the largest uniform field of view (FOV) of a sample on a multi scalable platform.

• 150×150 µm² field of view
• Illumination adaptable to the sample
• TIRF, HiLo or EPI illumination modes
• More quantitative data
• Homogeneous illumination, no interference patterns in the image

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SMLM Multicolor imaging

It is a powerful way to assess colocalization between different biological structures with highest resolution

All Abbelight platforms include various SMLM multicolor technologies:

  • Sequential multicolor imaging ​including fluidic exchange
  • Simultaneous multicolor imaging by using dichroic cubes including Spectral Demixing technology which is the most efficient way to image multiple structures:
    • Saves time by imaging two times faster
    • Avoids drift between the channels for most accurate colocalization information
    • No chromatic aberrations between the different structures imaged
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AbbelightUltimate 3D

Axial information with the highest resolution in x, y and z

To extract the 3D position of molecules with an ultimate precision and quasi-isotropy, Abbelight Enhanced 3D technology relies on a unique two-channel principle, by splitting the light on two different cameras. Our technology uses:

• One channel with a controlled astigmatism aberration that is induced by a custom cylindrical lens. This astigmatism is then measured to give access to the axial position of the emitting particle
• A second channel without any “modification” is used to preserve the best lateral resolution, enabling uncompromised access to the most accurate information possible in all the 3 axes

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