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Mitochondria Nanoarchitecture

Giulia Bertolin
Quantitative Fluorescence Microscopy


Fixed breast cancer cells (MCF7)


SAFe180, 3D STORM big FOV : 100 x 70 µm²

3D structural analysis

Mitochondria are often imaged using constitutive mitochondrial proteins, which are particularly abundant and therefore suitable for staining.  After fixation, primary antibodies were coupled to species-specific secondary antibodies, carrying fluorophores suitable for dSTORM. The use of dSTORM allows to study the localisation of mitochondrial proteins with greater precision than standard confocal microscopy.