Mitochondria Nanoarchitecture

Giulia Bertolin
Quantitative Fluorescence Microscopy

 

Fixed breast cancer cells (MCF7)

TOM22-AF647

SAFe180, 3D STORM big FOV : 100 x 70 µm²

3D structural analysis

Mitochondria are often imaged using constitutive mitochondrial proteins, which are particularly abundant and therefore suitable for staining.  After fixation, primary antibodies were coupled to species-specific secondary antibodies, carrying fluorophores suitable for dSTORM. The use of dSTORM allows to study the localisation of mitochondrial proteins with greater precision than standard confocal microscopy.