Podosomes
Liisa Hirvonen, Susan Cox
Randall Division of Cell and Molecular Biophysic
THP-1, fixed cells
Vinculin – iFluor-647
SAFe 180
THP-1 cells were seeded on fibronectin with TGF-β for 24 hours, then fixed with PFA. The cells were conjugated with a vinculin primary antibody and then a secondary antibody labelled with iFluor-647.