Ultrastructure of podosomes

Renaud Poincloux
Department of Molecular Mechanisms of Mycobacterial Infections, Phagocyte migration and differentiation

 

Unroofed Human macrophage, fixed

3D-dSTORM

F-actin-phalloidin

Talin-C, Talin-N, Paxillin AF 647

Density map

Spatial Organization

Height Distribution

Probability map

Using DONALD, they related force generation to the molecular extension of talin within the podosome ring, which requires vinculin and paxillin, indicating that the ring sustains mechanical tension.

Podosome Force Generation Machinery: A Local Balance between Protrusion at the Core and Traction at the Ring, Bouissou et al.; ACS Nano, 2017