Versatile wide field uniform illumination scheme, from classical fluorescence microscopy to single molecule localization microscopy

ABSTRACT :

Imaging and understanding of biological structures and dynamics require quantitative imaging, with the ability to label multiple components to assess their interactions. Here we present Adaptable Scanning for Tunable Excitation Regions (ASTER), a novel and versatile illumination technique that generates uniform illumination over adaptable fields of view, and is compatible with illumination schemes from epifluorescence to speckle-free TIRF. We quantify ASTER, notably in Single Molecule Localization Microscopy, and demonstrate its performance coupled with color-demixing schemes.

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